12 October 2012
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”I’m a Virgo,” Ford told me a week later

In these experiments, anti bclwav morpholinos were co injected together with an anti p53 morpholino (p53 MO) to abolish the non specific induction of p53 dependent cell death, a frequent off target effect of morpholinos17. Notably, two different morpholinos targeting distinct regions of the bclwav gene were used (bclwavMO1 and bclwavMO2), whereas a control morpholino (5mis MO, termed controlMO) was used as a negative control. The efficiency of the bclwavMOs was confirmed by immunoblotting of mitochondrial extracts of zebrafish embryos at 50% epiboly (Fig.

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